Lp-PLA2 FS Test Characteristics

  • Enzymatic test determining the activity of Lp-PLA2
  • Liquid-stable, ready-to-use reagents
  • Test is applicable on a wide range of fully automated analyzers
  • For use in serum, EDTA and heparin plasma
  • Wide measuring range up to 2000 U/L
  • 2-point calibration with superior stability of 8 weeks
  • No significant interferences by ascorbate, lipids, hemoglobin and bilirubin
  • Excellent precision over the entire measurement range

Interferences with bilirubin and hemoglobin

Activity of the analyte: 467 U/L
Limit of accepted unconjugated bilirubin interference: 61.3 mg/dL
Activity of the analyte: 932 U/L
Limit of accepted unconjugated bilirubin interference: 61.3 mg/dL
Activity of the analyte: 516 U/L
Limit of accepted hemoglobin interference: 1000 mg/dL
Activity of the analyte: 1019 U/L
Limit of accepted hemoglobin interference: 1000 mg/dL

Precision

Intra-assay
n = 20
Mean
[U/L]
 SD
[U/L]
CV
[%]
Sample 1  319  2.020.63
Sample 2  633  4.400.69
Sample 3 1113  7.980.72
Total precision CLSI
n = 80
Mean
[U/L]
 SD
[U/L]
CV
[%]
Sample 1  314  4.801.53
Sample 2  625  10.01.61
Sample 3 1105  13.31.20

Method comparison

Method comparison was performed against a competitor activity test. Passing/Bablok regression analysis between methods resulted in widely divergent results which may be explained by different standardizations. On average, competitor results are decreased by the factor 2.8. Using the obtained factor, values can be easily converted.